Novel function of osteoclastogenesis and bone resorption by membrane trafficking regulatory molecule

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Saga University
• Principal Investigator: Kukita Akiko 佐賀大学, 医学部, 准教授 (30153266)
• Co-Investigator(Kenkyū-buntansha): 久木田 敏夫 九州大学, 歯学研究院, 教授 (70150464)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 破骨細胞分化 / 小胞輸送 / 骨吸収

Outline of Final Research Achievements

Regulator of membrane trafficking play an important role in osteoclastogenesis and bone resorption. We analyzed expression and role of Nedd4 adaptor protein in differentiation and function of osteoclast. Nedd4 adaptor protein was induced in mononuclear osteoclast precursor cells by RANKL via p38MAPK pathway. Nedd4 adaptor protein was also expressed in osteoclasts formed on dentin slices. In addition, expression of the protein was detected in osteoclasts of bone tissues of adjuvant-induced arthritis rat. Knockdown experiment with shRNA lentivirus revealed that Nedd4 adaptor protein regulates transport of RANK to cell surface in osteoclast precursor cells. In addition, analysis of osteoclasts derived from knockout mice which were prepared using the CRISPR/Cas9 system showed that Nedd4 adaptor protein regulates bone resorption of osteoclasts.

Free Research Field

細胞生物学、骨代謝、分子生物学