Studies for expression and function of Runx2 isoforms

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KAWANE Tetsuya 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (00265208)
• Co-Investigator(Kenkyū-buntansha): 小守 壽文 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)
• Co-Investigator(Renkei-kenkyūsha): MIYAZAKI Toshihiro 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10174161) ; MORIISHI Takeshi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (20380983)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Runx2 / isoform / 骨・軟骨

Outline of Final Research Achievements

Runx2 is the essential transcription factor for osteoblast differentiation and latter term of chondrocyte differentiation involved in bone formation. Runx2 is expressed as two isoforms (type I and type II) encoded by two different mRNAs with distinct 19 N-terminal amino acids sequence. The type I and type II Runx2 are expressed from the upper stream promoter (P2) and downstream promoter (P1), respectively. In this study, we investigate the regulation of Type I Runx2 expression through prenatal period in mice. As a result, strong expression were detected in osteoblasts and expanded cartilage layer chondrocytes, although little signal was detected in hypertrophic chondrocytes. Therefore, these two isoforms were regulated by different manner.

Free Research Field

細胞生物学　生化学