2017 Fiscal Year Final Research Report
Exploration of RNA network between tissues in fetal mouse salivary gland
| Project/Area Number |
15K11060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
林 徹 北里大学, 医療衛生学部, 講師 (10454266)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | マイクロRNA / 唾液腺 / 上皮間葉相互作用 |
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| Outline of Final Research Achievements |
Mouse salivary glands at embryonic day 13 (E13) have been a model organ to study epithelial-mesenchymal interactions. We reported that mesenchymal-derived microRNAs (miRNAs) in the salivary gland are transported to the epithelium. The miRNAs transported from the mesenchyme could target multiple RNAs such as mRNA and long non-coding RNA in the epithelium. Therefore it is hypothesized that those RNAs build an RNA network and influence the expression level of each other by competing for miRNA binding. To explore the RNA network between tissues during salivary gland organogenesis, miRNA transport from the mesenchyme to the epithelium was inhibited and then transcripts in the epithelium was analyzed with RNA-seq. The data showed up-regulation of 27 transcripts expression levels by inhibition of miRNA transport, suggested that these miRNAs and transcripts comprise an RNA network between tissues in E13 mouse salivary glands.
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| Free Research Field |
薬理学、発生学
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