Role of microenvironment on maintenance of odontoblasts: analysis by using novel odontoblast-isolation method

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SHIBATA Yasuaki 長崎大学, 医歯薬学総合研究科(医学系), 講師 (80253673)
• Co-Investigator(Kenkyū-buntansha): 池田 通 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00211029) ; 中島 和慶 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (40707246)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 象牙芽細胞 / 培養 / Tie2 / Angiopoietin-1 / 微小環境 / エナメル器

Outline of Final Research Achievements

The purpose of this study is to explore the role of microenvironment in maintenance of odontoblast differentiation. We isolated the odontoblast fraction by using Tie2 and CD31 antibody. Expression of differentiation markers in monoculture of the odontoblasts decreased within 1 week, whereas those were maintained when the odontoblasts was co-cultured with the mesenchymal fraction (Tie2-). These results revealed that the microenvironment of the dental pulp is essential for the maintenance of the odontoblast differentiation. In parallel with the study, we explored the expression of Angiopoietin-1 (Ang-1) in mouse enamel organ. Ang-1 was expressed in enamel organ in associated with the invasion of Tie2-positive capillaries at embryonic day 17. Our result suggested that Ang-1/Tie2 system regulates not only odontoblast differentiation but also involution of enamel organ.

Free Research Field

顕微解剖学