Synergistic network on COX-2 production by PAR-1 activation cascade in pulp

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: KAMIO Naoto 日本大学, 松戸歯学部, 助教 (10508774)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヒト歯髄細胞 / カリクレイン / PAR-1 / COX-2 / 炎症

Outline of Final Research Achievements

The purpose of this study is to clarify the mechanism of COX-2 production via PAR-1 activation by kallikrein in human dental pulp cultured cells, and the interaction network of kallikrein and plasmin and bradykinin.
Kallikrein increased the expression of COX-2 mRNA in a concentration- and time-dependent manner. Kallikrein promoted the production of COX-2 protein, and this effect was suppressed by PAR-1 inhibitor.
In the search for the interaction by plasmin · kallikrein · bradykinin, the effect was not confirmed by intracellular Ca2+ concentration measurement. However, the stimulation of kallikrein and bradykinin enhanced the expression of COX-2 protein compared to when stimulated alone.

Free Research Field

医歯薬学