The surface morphology of titanium plate could change macrophage activity.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: OKUYAMA YAYOI 東北大学, 大学病院, 助教 (30223697)
• Co-Investigator(Kenkyū-buntansha): 江草 宏 東北大学, 歯学研究科, 教授 (30379078) ; 新部 邦透 東北大学, 大学病院, 助教 (50468500)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 顎堤吸収 / チタン表面性状 / 破骨細胞 / 骨芽細胞

Outline of Final Research Achievements

Different surface textured titanium plate such as mechanical polishing surface (MS) and acid treatment surface (AS) were prepared. RAW 264.7 cells known as osteoclast precursor cells were cultured on each surfaces, and their gene expression was examined. As a result, the expression of NOS2, a marker of inflammatory macrophages (M1 macrophages), increased in RAW 264.7 cells cultured on the MS surface. On the other hand, RAW 264.7 cells cultured on the AS surface showed similar to macrophages cultured on polystyrene (negative control). RAW 264.7 cells were cultured on the MS surface and the AS surface,and after 72 hours the culture supernatant was collected. By continuing detailed studies in the future, we plan to clarify how titanium surface properties affect macrophage activity.

Free Research Field

補綴理工系歯学