Study on minimally invasive tissue sterilization in regenerative medicine using atmospheric pressure low temperature plasma treatment

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K11229
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tsurumi University
• Principal Investigator: OHSHIMA Tomoko 鶴見大学, 歯学部, 学内教授 (50233101)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: プラズマ医療 / 感染根管モデル / プラズマ処理水 / 殺菌効果 / 生体安全性

Outline of Final Research Achievements

This study aimed to evaluate atmospheric low temperature plasma as a basic research for the development of technology that can non-invasively sterilize regenerated transplanted tissues and host recipient tissues. It was verified that the active species produced by the plasma show sufficient bactericidal activity and that the activity disappears in a short time and does not show residual activity.
In the verification of the infection model, plasma treated water showed a very high effect in a shorter time than the irradiation method. As a result of conducting a safety test of plasma treated water in a graft recipient tissue and transplanted cell (monolayer cell and graft tissue sheet) model, no metabolic activity failure was observed, and high safety was shown.
Therefore, it was concluded that plasma-treated water has high bactericidal activity and biological tissue safety.

Free Research Field

口腔微生物学

Academic Significance and Societal Importance of the Research Achievements

日本の再生医療は臨床での実用化レベルに達しており、再生組織のベースとなるiPS 細胞が高率に分離される歯髄の使用が増加すると思われるが、口腔からの感染と隣り合わせである。しかし既存の消毒薬は組織の損傷・細胞毒性が懸念されるため使用できず、抗菌薬に依存しているため、抗菌薬アレルギー患者は適応外とされているが、そのような症例も等しく再生医療を受けられる必要性に迫られると予想される。プラズマ処理水に含まれる活性種は、検出限界以下まで殺菌する一方で、ほぼ組織毒性を示さないことが今回の研究で明らかとなった。したがって再生医療のみならず、広く医療全般での有用性が期待される。