2017 Fiscal Year Final Research Report
Regenerative effect of dentin and periodontal tissue by cytokine therapy induced due to Er:YAG laser combination
| Project/Area Number |
15K11233
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tsurumi Junior College |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Er:YAGレーザー / hPDL細胞 / 歯髄細胞(組織) / TGF-β / MMP / プラスミン / HSP |
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| Outline of Final Research Achievements |
Transforming growth factor-beta (TGF-β), which is an important cytokine that controls cell proliferation and differentiation, was activated in hPDL cells by Er:YAG laser irradiation. However, the direct activation was not done, and MMP-2, MMP-3, MMP-8 gene expression was involved in its activation, suggesting the involvement of HSP90α.
Er:YAG laser irradiation on the porcine pulp tissue had an effect of increasing ALP activity, and MMPs activity of 53,45,40 kDa and non-MMP activity of 33 kDa were observed. Enhancement of MMP-2 gene expression was recognized by laser irradiation on immortalized cells (PPU-7 cells) derived from porcine pulp tissue, and it was considered to be involved in TGF-β activity.
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| Free Research Field |
歯科医用工学・再生歯学
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