Investigation of reguratory system of VEGF-VEGFR signaling via VEGFR2 by anti-bone modifying agents

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima (2017); Hiroshima University (2015-2016)
• Principal Investigator: NAKAGAWA Takayuki 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (30456230)
• Co-Investigator(Kenkyū-buntansha): 武知 正晃 広島大学, 医歯薬保健学研究科(歯), 准教授 (00304535) ; 太田 耕司 広島大学, 病院(歯), 助教 (20335681)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 薬剤関連顎骨壊死

Outline of Final Research Achievements

Mononuclear cells were isolated from peripheral blood by the density gradient method and CD11b positive cells (CD11b +) were further extracted by the magnetic cell sorting method. Osteoclast differentiation was induced in CD11b + cells by RANKL. Further administration of zoledronic acid inhibited the formation of osteoclasts of CD11b + cells, and the expression of NFATc1 and VEGFR2, which are osteoclast differentiation markers, also declined. Furthermore, in order to investigate the effect of differentiation of osteoclasts of VEGFR2, administration of VEGFR2 neutralizing antibody and differentiation of osteoclasts resulted in inhibition of osteoclast differentiation in a concentration dependent manner by administration of neutralizing antibody.

Free Research Field

口腔外科学