Analysis of cellular endocrinological properties of CD133-positive oral cancer-derived sphere in a serum-free suspension culture system

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11251
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Toratani Shigeaki 広島大学, 医歯薬保健学研究科(歯), 准教授 (90172220)
• Co-Investigator(Kenkyū-buntansha): 岡本 哲治 広島大学, 医歯薬保健学研究科(歯), 教授 (00169153)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口腔がん / がん幹細胞 / CD133

Outline of Final Research Achievements

In this study, we intended to develop a novel diagnostic and therapeutic method targeting cancer stem cells of human oral cancer and performed cellular and molecular biological characteristics analysis of CD133 positive cell group isolated from oral squamous cell carcinoma cell (OSCC) in serum - free culture system.
As a result, there was a transit mechanism from CD133 positive cells to negative cells or from CD133 negative cells to positive cells in OSCC. EGF and SHH were thought to promote transit from CD133 negative cells to positive cells. On the other hand, anticancer agent and hypoxic environment targeted only CD133 negative cells, and transit from negative cells to positive cells was considered not to be inhibited. It seems that the EGF and SHH pathways play an important role in maintaining the function of the positive cell OSCC.

Free Research Field

口腔外科学