Development of human periosteal-derived cell sheets cultured on an amniotic membrane substrate for bone regeneration.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11261
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Kanamura Narisato 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70204542)
• Co-Investigator(Kenkyū-buntansha): 雨宮 傑 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90398389) ; 山本 俊郎 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472) ; 本城 賢一 京都府立医科大学, 医学(系)研究科(研究院), 特任助教 (00756877) ; 足立 哲也 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10613573)
• Research Collaborator: SOTOZONO Chie ; NAKAMURA Takahiro ; ADACHI Keiji
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 移植・再生医療 / 再生医学 / 細胞・組織 / 歯学

Outline of Final Research Achievements

Periosteal-derived cells (PDCs) were cultured on AM to generate novel periosteal cell sheets, and the kinetics of the cell sheets were immunohistochemically investigated in vivo. The AM was collected from the placenta during a caesarean section. For PDCs, primary culture of the periosteal tissue was passaged 3-4 times. These cells were cultured on AM. The resulting culture sheets were transplanted under the renal capsules of nude mice, and were removed after 4 weeks.
PDCs proliferated on AM, forming single cell sheets, which demonstrates that AM is suitable as a culture substrate. The periosteum contains cells that have the ability to differentiate into osteoblasts and osteocytes. The cell sheets expressed osteoblast proteins, which were maintained even in an in vivo environment.

Free Research Field

歯学・外科系歯学