Challenge to inferior alveolar nerve regeneration by human iPS cells for feeder-free and xeno-free system

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: Kawana Hiromasa 慶應義塾大学, 医学部(信濃町), 准教授 (50224803)
• Co-Investigator(Kenkyū-buntansha): 森川 暁 慶應義塾大学, 医学部(信濃町), 講師 (00424169)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 末梢神経再生 / 神経損傷

Outline of Final Research Achievements

The present study aims to induce neural crest cells from iPS cells and restore function of damaged nerve tissue. We established iPS cell culture method with feeder-free, xeno-free system for inducing neural crest cells. In order to investigate whether the induced neural crest cell population actually has the potential to restore peripheral nerve injury, we started the creation of the sciatic nerve cutting model and its functional evaluation. We found that transplanted induced neural crest cells possess the properties of mesenchymal stem cells and vascular endothelial cells that could also be induced into regenerating nerve tissues. These cells expressed peripheral nerve markers in transplantation tissues.

Free Research Field

口腔外科学