Cell sheets were prepared and layered to produce a hybrid cell sheet with verification of transplant

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Abe Shinichi 東京歯科大学, 歯学部, 教授 (40256300)
• Research Collaborator: Yamane Shigeki ; Umezawa Takashi ; Serikawa Masamitsu
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 筋芽細胞 / Desmin / IGF-1 / コラーゲン / 頬粘膜

Outline of Final Research Achievements

Cell sheets of myoblasts and mesenchymal cells were generated, and the effects of the mesenchymal cells on the myoblasts during long-term cultivation were investigated. Presence of desmin and collagen type IV seen in normal muscle tissues was also confirmed in the produced cell sheets. IGF, insulin-like growth factor, was expressed only when the mesenchymal cells and myoblasts were co-cultured, suggesting the presence of mesenchymal cells in the long-term co-culturing system could influence myoblasts proliferation. Furthermore, the cell sheet was transplanted into the rabbit lamina mucosa, and the localization of the muscle specific protein (desmin) and collagen was observed, and it was confirmed that the cell sheet was assimilated in the mucosa.

Free Research Field

組織解剖学