Study on establishment of new periodontal ligament stem cell isolation method using RNA detection probe

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: Kawanabe Noriaki 岡山大学, 医歯薬学総合研究科, 准教授 (00397879)
• Co-Investigator(Kenkyū-buntansha): 片岡 伴記 岡山大学, 医歯薬学総合研究科, 助教 (50580180) ; 住吉 久美 岡山大学, 医歯薬学総合研究科, 助教 (80625161) ; 中村 政裕 岡山大学, 大学病院, 助教 (20708036)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヒト歯根膜幹細胞 / RNA検出プローブ

Outline of Final Research Achievements

The purpose of this research is to investigate RNAs specifically expressed in human periodontal stem cells, and to establish a method to efficiently isolate human periodontal stem cells as living cells using RNA detection probes. As a result, LRIG1 was identified as one of the RNAs specifically expressed in human periodontal stem cells. In addition, as a result of comparison between LRIG1 positive and LRIG1 negative human periodontal ligament cells using LRIG1 RNA detection probes, LRIG1 positive human periodontal ligament cells have higher cell proliferative capacity, and adipogenic and osteogenic differentiation capacity in vitro was also high. Therefore, it was suggested that LRIG1 is useful for identification of human periodontal stem cells.

Free Research Field

矯正歯科