2017 Fiscal Year Final Research Report
Effects of cathepsin A on the regeneration of periodontal ligament tissue
| Project/Area Number |
15K11385
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山田 聡 東北大学, 歯学研究科, 教授 (40359849)
山下 元三 大阪大学, 歯学部附属病院, 講師 (90524984)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | カテプシンA / Ebelactone B / 歯根膜細胞 / 歯周組織再生 / ゲノムワイドアプローチ |
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| Outline of Final Research Achievements |
The purpose of this study is to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.
Mouse periodontal ligament cells (MPDL22) were incubated with cathepsin A inhibitor Ebelactone B and calcification-related gene expression was assessed by real-time PCR. As a result, Ebelactone B clearly inhibited the expression of calcification-related genes such as osteopontin and type I collagen. We next evaluated genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population through exome sequencing. One of a single nucleotide polymorphism (SNP) in cathepsin A, rs181943893, showed significant different minor allele frequency (MAF) through a case-control exome sequencing.
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| Free Research Field |
歯周病学
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