Growth inhibition of selected bacterial species by peptide nucleic acids for control of oral microflora

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Osaka Dental University
• Principal Investigator: MAEDA Hiroshi 大阪歯科大学, 歯学部, 教授 (00274001)
• Co-Investigator(Kenkyū-buntansha): 苔口 進 岡山大学, 医歯薬学総合研究科, 准教授 (10144776) ; 高柴 正悟 岡山大学, 医歯薬学総合研究科, 教授 (50226768) ; 北松 瑞生 近畿大学, 理工学部, 講師 (60379716)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細菌叢 / ペプチド核酸 / アンチセンス

Outline of Final Research Achievements

In this research project, we aimed to inhibit the growth of selected bacterial species in oral microflora by using antisense PNA (peptide nucleic acids). By targeting the HSP and AcpP genes of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, the antisense PNAs were designed and synthesized with carrier paptide (KFFKFFKFFK). For P. gingivalis, both antisense PNAs effectively inhibited the growth. Especially, anti-HSP PNA completely inhibited the growth for 5 hours. Comparing to P. gingivalis, the inhibit effect was weak for A. actinomycetemcomitans. Anti-HSP PNA slightly inhibit the growth, while anti-AcpP PNA did not show the effect. The cause of the low effect may be due to the small uptake of the PNA in A. actinomycetemcomitans. Antisense PNA may have the potential to reduce the population of P. gingivalis in oral microflora. New designs for carrier peptide and the target genes will be required for A. actinomycetemcomitans.

Free Research Field

微生物学