2017 Fiscal Year Final Research Report
Development of the analyzing system of DNA polymorphism aiming at application to the highly degraded samples for personal identification in Forensic Odontology
| Project/Area Number |
15K11455
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Social dentistry
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | DNA多型 / 戦没者遺骨 / 高度変性資料 / ミトコンドリアDNA型 / Y染色体多型 / X染色体STR / 硬組織 / DNA抽出 |
|---|
| Outline of Final Research Achievements |
This study aimed at solution of two kinds of difficulties in the DNA analysis of the dead in WWII; one of them was identification of distant blood relationship between the dead and their relatives and the other was DNA typing from highly degraded DNA samples. Following results were obtained. We established a method that can determine mtDNA types from highly degraded bone or tooth samples common to southern area. The number of phylogenetic mtDNA haplogroups lineages at the basal level in the Japanese and Malay populations was increased. We constructed a system which can evaluate the identity of Y-STRs between the dead and their distant blood relative. A method to determine Y-lineage from highly degraded samples was also developed. The number and kinds of closely-linked X-STR haplotypes were increased. These were applied to elucidate complicated cases. A method to isolate amplifiable DNA from highly degraded samples was developed and was applied in actual forensic cases.
|
| Free Research Field |
歯科法医学
|
