Construction of RNA based life-like chemical system for Darwinian evolution on the basis of the replication reaction consisting of three base units

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12144
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Life / Health / Medical informatics
• Research Institution: Hiroshima Shudo University
• Principal Investigator: Kawamura Kunio 広島修道大学, 人間環境学部, 教授 (50204772)
• Research Collaborator: Maurel Marie-Christine University Pierre and Marie CURIE, Institutde Systématique, Evolution, Biodiversité, 教授 ; KONAGAYA NORIKO 安田女子大学, 家政学部, 准教授 ; MARUOKA YOSHIMI
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: RNAワールド / 鋳型指示反応 / オリゴヌクレオチド / 化学反応速度論 / 生命の起源 / 化学進化 / アストロバイオロジー / 原始地球

Outline of Final Research Achievements

We attempted to make an RNA replication model on the basis of three base unit template-directed reactions, which would lead a new experimental approach for spontaneous chemical reaction system in vitro. Using two types of template-directed reactions, which are the regular template-directed reaction (TD reaction) and a template-direcetd reaction including 2 - 6 members of nucleoside bases (S-TD reaction). The influence for these TD reactions, (1) influence of protein-like molecules, (2) the influence of sequences of S-TD reaction, (3) the influence of zinc ions, (4) HPLC analytical method for the evaluation of these models have been investigated. According to these analyses, we emphasize that we need to make a conclusion which would be conflict to the RNA world hypothesis since the replication of RNA could hardly started under the primitive Earth conditions.

Free Research Field

生命の起源，分析化学，生体分子の化学，熱水反応