2016 Fiscal Year Final Research Report
Quantitative analysis of tissue and cellular dynamics during early forebrain formation
Project/Area Number |
15K12147
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Life / Health / Medical informatics
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
OHTSUKA DAISUKE 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (40632865)
|
Co-Investigator(Renkei-kenkyūsha) |
MORISHITA YOSHIHIRO 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (00404062)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Keywords | 形態形成 / 発生動態 / 器官形成 |
Outline of Final Research Achievements |
Brain morphogenesis begins with complex 3D deformation of a simple cylindrical structure called neural tube that is composed of monolayer neuroepithelium in earlier stages. As is the case for many organs, “how microscopic molecular and cellular dynamics are linked to macroscopic organ morphology” remains an open question. Here we focus on the optic vesicle evagination process that is a main morphogenetic event during early forebrain morphogenesis. We constructed tissue-level 3D deformation maps for chicken forebrain morphogenesis. The tissue deformation analysis showed that globally-aligned anisotropic deformation along the medio-lateral axis is the predominant morphogenetic mechanism that occurs throughout the entire period of our focus. To clarify the underlying molecular/cellular mechanisms of this directional tissue stretch, we quantified cellular dynamics and characteristics. These analyses indicated that tissue-level anisotropic deformation is driven by cell rearrangements.
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Free Research Field |
発生生物学
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