2017 Fiscal Year Final Research Report
Towards the diagnosis method for the toxicity of organophosphorus
| Project/Area Number |
15K12215
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
梶原 景正 東海大学, 医学部, 講師 (00204397)
坂部 貢 東海大学, 医学部, 教授 (70162302)
加藤 明 東海大学, 医学部, 准教授 (70546746)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 有機リン / シックハウス症候群 / 神経障害エステラーゼ / 複合体解析 |
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| Outline of Final Research Achievements |
Sick Building Syndrome(SBS) is caused by volatile organic compounds and organophosphorus(OP). However, despite the presence of SBS symptoms in patients the actual pathogenesis has not yet been developed.
Since OP covalently bind Neuropathy Target Esterase (NTE) we construct the transgenic mice carrying human PNPLA6 cDNA which codes human NTE. The skin of the mice could digest the ester bond specific for human NTE and showed the atrophy of pyramidal cells in hippocampus and the Purkinje cells in cerebellum after exposure for high dose formalin. We also developed the new mutant mice of pnpla6 gene using CRISPR/ Cas9 system. Exposure of OP to chick embryos cause hemorrhage at the top of the brain. The NTE expression in a human brain died by drinking OP decreased and paralysis and aggregation of granular cells in the cerebellum occurred.
Using human PNPLA6 cDNA, we made the part of NTE which contain active site in E.coli cells and human cells and partial purification was completed
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| Free Research Field |
分子生物学
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