2016 Fiscal Year Final Research Report
Diversity and harmonization of expressed functions in microbial ecosystems
| Project/Area Number |
15K12228
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental engineering and reduction of environmental burden
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 微生物生態系 / 微生物間相互作用 / 微生物動態 / 増殖 / 微生物群集 / 合成微生物生態系 / 制御 / 数理モデル |
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| Outline of Final Research Achievements |
This study investigated the factors that determine the dynamics of bacterial communities in a complex system using multidisciplinary methods. Three phenol-degrading, phylogenetically different bacterial strains were used. Two metrics, J parameter based on kinetic parameters and ‘interspecies interaction’, were compared to predict which strain would become dominant in a SME. Population densities of strains used in SMEs were enumerated by real-time PCR techniques and were compared to predictions made from those two metrics. These results demonstrate that the effects of interspecies interactions within microbial communities contribute to determining the dynamics of the microbial ecosystem. The supernatant of strain C8 (C8SP) repressed significantly the growth of strains R2. Of the C8SP fractionated with different molecular weight and organic solvents, the fraction less than 500 Da in the H2O-soluble components repressed the growth of strain R2.
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| Free Research Field |
微生物生態学
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