2017 Fiscal Year Final Research Report
Development of a genetically engineered microorganism able to grow on trichloroethene and its application to the treatment
| Project/Area Number |
15K12239
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | トリクロロエチレン / 分解処理 / フェノールヒドロキシラーゼ / グリオキシル酸 |
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| Outline of Final Research Achievements |
Development of a genetically engineered microorganism (GEM) able to grow on trichloroethene (TCE) was carried out for cleanup a TCE contaminated site aerobically. Phenol hydroxylase (PH) is known to degrade TCE completely via glyoxylate. We tried to develop a new GEM that degrade TCE efficiently. After analyzing TCE degradability of PH genes, a new hybrid PH gene made from 2 different PH genes was developed to make a GEM that could degrade TCE efficiently. Also a Cupriavidus bacterium was used as a host of the gene, because that bacterium was able to use glyoxylate as a sole carbon source for growth. However, we did not succeeded in decreasing toxicity of TCE epoxide by introducing etnE that metabolize vinyl chloride (VC) epoxide.
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| Free Research Field |
環境生物工学
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