2016 Fiscal Year Final Research Report
Genetic engineering for biosynthesis of copolymer from carbon dioxide
| Project/Area Number |
15K12267
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Prefectural University of Kumamoto |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Kenji 近畿大学, 産業理工学部, 教授 (20236582)
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| Research Collaborator |
WAKIDA Izumi
KURADOMI Yuki
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | ポリヒドロキシアルカン酸 / 生分解性プラスチック / 脂肪酸合成経路 / 二酸化炭素 / 共重合ポリエステル / コポリマー |
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| Outline of Final Research Achievements |
The (R)-3HA-CoA ligase gene in Pseudomonas sp. 61-3 was cloned and identified. The gene was encoded the protein composed of 560 amino acids with a calculated molecular mass of 62 kDa. PHA synthase gene (phaC1) and the genes encoding the enzymes which provide medium-chain-length 3HA unit through fatty acid biosynthesis pathway were introduced into Ralstonia eutropha H16dC (phbC -disruptant of H16). The recombinant strains accumulated 5-10 wt% P(3HB-co-3HA) with 5-6 mol% 3HA fractions from fructose as the sole carbon source. Whereas, the recombinants synthesized less than 2 wt% P(3HB) homopolymer from carbon dioxide and 3HA units were not incorpotaed the polymer chain.
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| Free Research Field |
応用微生物学
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