Preparation of materials carrying with glycans and titanium dioxide to capture and eliminate specific pathogens

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12314
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Clothing life/Dwelling life
• Research Institution: Nagaoka University of Technology
• Principal Investigator: FURUKAWA Kiyoshi 長岡技術科学大学, 工学研究科, 名誉教授 (10190133)
• Research Collaborator: NISHIKAWA Masami
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 酵母ペースト / 選択的捕捉 / 大腸菌 / 酸化チタン / 光触媒 / 殺菌

Outline of Final Research Achievements

A variety of toxic microorganisms such as bacteria and viruses bind to glycans with respective structures at cell surfaces. We prepared the filters and beads carrying glycans to trap bacteria. However, such materials can trap microorganisms but cannot eliminate them. In the present study, we first prepared a slide glass coated with titan dioxide on which yeast pastes were mounted in a disk shape. When it was incubated with fluorescence-labeled E coli, the bacterium was trapped on the yeast pastes showing a fluorescent disk with a diameter of 3.7 mm long on the glass. Then, it was irradiated with a black-lamp (UV) for 2 hours at room temperature, and the size of the fluorescent disk was 2.8 mm long. These results indicate that E. coli trapped at the peripheral zone of the yeast pastes was killed with hydroxyl radicals generated from titan dioxide by UV-irradiation, showing that the combination of glycans with titan dioxide is useful to eliminate specific pathogenic organisms.

Free Research Field

糖鎖工学