Isolation, proliferation and differentiation of human adult pancreatic stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12506
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: Hasegawa Kouichi 京都大学, 物質－細胞統合システム拠点, 特定拠点講師 (50378890)
• Research Collaborator: PERA Martin F メルボルン大学, 教授 ; FARLEY Alison M メルボルン大学, 研究員 ; VARTAK-SHARMA Neha インド国立幹細胞生物学再生医学研究センター(inStem), 研究員 ; NAYER Bhavana インド国立幹細胞生物学再生医学研究センター(inStem), 研究員
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 膵臓 / 組織幹細胞 / 再生医療

Outline of Final Research Achievements

Human adult pancreas has been thought not to have regeneration ability. Recently, putative quiescent pancreatic stem/progenitor cells were discovered. However, study of the cells is still limited because the method for isolating living progenitors is not developed.
We have previously developed an antibody which can mark surface of the progenitors in endodermal organs including pancreas. In this study, we discovered that the antibody marks progenitors in pancreas, and developed efficient methods to isolate the progenitors. Utilizing comprehensive gene expression analysis, we also identified several signaling pathways may involve in growth and differentiation of the progenitors. We are examining effect of chemical compounds which can modify the signaling pathways.

Free Research Field

幹細胞生物学