Microbiomechanics of glioma stem cells representing tumor heterogeneity in their invasion process

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12521
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Keio University
• Principal Investigator: SUDO RYO 慶應義塾大学, 理工学部(矢上), 准教授 (20407141)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 3次元浸潤モデル / 間質流 / 共培養 / グリオーマ幹細胞

Outline of Final Research Achievements

This project aimed to investigate 3D invasion process of glioma stem cells (GSCs) coculturing with endothelial cells (ECs) using a microfluidic device. In particular, we focused on microenvironmental factors such as differentiation status of GSCs, interstitial flow, and interactions with blood vessels, to investigate control factors of GSC invasion. Our results revealed following things. 1) GSCs in differentiation conditions significantly responded to interstitial flow application compared with those in stem cell conditions. 2) GSCs extended characteristic protrusions downstream of interstitial flow, resulting in an increased invasion distance. 3) GSCs acquired invasion activity in the presence of ECs.

Free Research Field

細胞バイオメカニクス