Investigation of new materials introduction method into cells using filament matrix

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K12529
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute for Materials Science
• Principal Investigator: Kobayashi Hisatoshi 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクト二クス研究拠点, 上席研究員 (90354266)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 単繊維 / 新規遺伝子導入ツール / 流路 / 細胞走化性

Outline of Final Research Achievements

In this study, we tried to develop the new transfection device based on the nanofiber-cell strong interaction and using the 1D continuity of fiber and chemotaxis of the cell. Flow chamber system utilized FBS as chemotactic factor was developed. Using the above system, gene introduction to GFP-Hela cell on a filament was evaluated by analyzing the quenching of GFP by using GFP-siRNA as transgene. In the current status, cell spreading on the filamental substrate and quenching of the GFP were observed, but, apotosis was also observed, targeted high efficiency system is not developed yet. We will push forward further examination in future.

Free Research Field

生体材料学