Investigation of the mechanism of gene transfer by sonoporation for fetus

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12530
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute of Maritime, Port and Aviation Technology (2016); Port and Airport Research Institute (2015)
• Principal Investigator: SATO Tomoo 国立研究開発法人海上・港湾・航空技術研究所, 港湾空港技術研究所, 専任研究員 (10242918)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ソノポレーション / 胎児治療 / 遺伝子導入 / 超音波 / マイクロバブル / 実験計画法 / 緑色蛍光タンパク質 / 蛍光画像

Outline of Final Research Achievements

There is a great concern about the gene therapy to fetuses. We pay attention to sonoporation which is method using ultrasound and micro-bubbles to transfer the gene into target organ (cell) selectively. The purpose of our study is to build the method of gene-transfer that is more efficiently and more safety, by studying the dynamics of the cell membrane during sonoporation. We tried development of the technique to observe the dynamics of the cell membrane using super high spatio-temporal resolution confocal microscope with high sensitivity.

Free Research Field

超音波工学