Hunting of novel myokines by RNAseq

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12668
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Fujii Nobuharu L 首都大学東京, 人間健康科学研究科, 教授 (40509296)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 骨格筋 / RNAseq / マイオカイン

Outline of Final Research Achievements

Messenger RNA obtained from human skeletal muscle tissue was provided to Next Generation Sequence (RNAseq) to identify gene transcripts exhaustively. The transcripts were compared to human genome information to seek transcripts that had not reported yet. Then, secretion protein-like molecules were estimated from amino acid sequence of the transcripts. Finally, we found 3 secretion protein-like molecules expressed in skeletal muscle. One of the 3 was focused in the subsequent study. Unfortunately we did not achieve susses to make a specific antibody for the molecule. Therefore HA-tagged expression vector of the molecule was generated and expressed in cultured skeletal muscle cells. The expressed protein was detected in conditioned culture medium by Western blotting used HA-antibody. These results suggest that the molecule is a novel myokine secured from skeletal muscle.

Free Research Field

代謝内分泌科学　運動分子生物学