Generation of human skeletal muscle in the muscles of immunodeficient mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12675
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2016); Fujita Health University (2015)
• Principal Investigator: Uezumi Akiyoshi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 専門副部長 (60434594)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 筋衛星細胞 / 細胞表面マーカー / ヒト細胞 / 移植

Outline of Final Research Achievements

Skeletal muscle contains stem cell population called satellite cells. Satellite cells can generate myotube in vitro but there is a critical difference between myotube and skeletal muscle tissue. In addition, use of human cells is needed for the medical research. It is possible that making human muscle fibers in vivo by transplanting human satellite cells into the muscles of immunodeficient mice. However, it is difficult to achieve efficient transplantation of human satellite cells because purification method of human satellite cells has not been established. In this study, we discovered novel marker of human satellite cells, CD82. We demonstrated that efficient transplantation of human satellite cells into immunodeficient mice can be done by utilizing CD82 as a positive selection marker. We believe that our results provide useful information for the study of human skeletal muscle cells.

Free Research Field

骨格筋生物学