Site Specific Antibody Modification Using Catalyst-Functionalized Affinity Beads

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K12742
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Sato Shinichi 東京工業大学, 科学技術創成研究院, 助教 (20633134)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 触媒的タンパク質修飾 / ラジカル反応 / アフィニティー精製 / タンパク質機能化 / Ru光触媒 / 抗体化学修飾

Outline of Final Research Achievements

We developed the protein modification technique which take place in the proximity of a few nanometers around the ruthenium photocatalyst. The two types of small molecules, the Ru / dcbpy complex and the ligand molecule of the target protein, were modified on the beads. This ruthenium photocatalyst-functionalized affinity beads enable the target selective purification from protein mixture and chemical labeling simultaneously without significant non-specific binding proteins to the beads. In addition, (1) the high sensitive detection of ligand binding protein with chemical labeling, (2) the detection of low affinity ligand binding protein that is difficult to be detected by conventional affinity chromatography method, (3) antibody purification and chemical labeling were achieved.

Free Research Field

化学生物学、有機化学、創薬化学