Creation of Novel Thiochromone-Caged Compounds and their Application to Developing New Cell Sorters

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K12748
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Kakiuchi Kiyomi 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (60152592)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 生体分子 / ケージド化合物 / チオクロモン誘導体 / アンチセンスオリゴ核酸 / シクロデキストリン / コレステロール

Outline of Final Research Achievements

We aimed to synthesize and functionalize novel caged compounds, to which the thiochromone-type photolabile protecting group (TCM) is introduced. This TCM can protect various alcohols, amines, and phosphoric acids, and can be converted to a highly fluorescent compound after the uncaging process. We synthesized thymidine, resveratrol, and beta-cyclodextrin caged compounds with our TCM. Photoirradiation, namely uncaging, of these TCM-caged thymidine, resveratrol, and beta-cyclodextrin led to the recovery of their original compounds, respectively. Recovered compounds showed their own biological activity. Totally, we could demonstrate that the TCM protecting group is effective for the creation of various types of caged compounds.

Free Research Field

ケミカルバイオロジー