Development of molecular probe for GST

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12751
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Nagoya University
• Principal Investigator: Abe Hiroshi 名古屋大学, 理学研究科, 教授 (80415067)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 酵素 / 生理活性 / イメージング

Outline of Final Research Achievements

Glutathione S transferase (GST) is one of highly expressed enzymes in cancer cells. The subtype of this enzyme depends on the cancer species. Tough the determination of the cancer species by detecting GST subtype is thought to contribute to cancer therapy, the GST probe with subtype specificity remains to be developed. As fundamental step for developing the subtype-specific probe, glutathione derivatives were designed and synthesized and those subtype specificities were evaluated.
We synthesize glutathione derivatives with fluorescent group or the functional group forming covalent bond with amino-acid residue. Using this derivative, we discovered the structure for the subtype-specificity. In addition, we succeeded in a synthesis of derivatives with several chemical groups at glycine residue in glutathione without reduction of GST binding ability. These results are thought to promote the development of probes with GST substrate specificity.

Free Research Field

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