Desing method of intracellular RNA-protein structure

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13314
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: Fujita Yoshihiko 京都大学, iPS細胞研究所, 特定拠点助教 (60589266)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: RNA / synthetic biology / nano technology

Outline of Final Research Achievements

We succeeded in controlling the cellular function depending on the intracellular environment by designed artificial RNA that interacts with endogenous protein and forms RNA-protein nanostructures. We also succeeded in constructing an RNA switch whose translational level increased in response to the activity of intracellular miRNA. Both are fundamental technologies for controlling the cellular functions. Notably, designed miRNA-responsive RNA switches enabled us to remove specific cells without a cell sorter. This result indicates the possibility of medical applications such as removal of cancer cells and undifferentiated.

Free Research Field

RNA synthetic biology