High-density packaging of nano-devices using DNA highwires as scaffolds

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K13324
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Kagawa University
• Principal Investigator: TERAO Kyohei 香川大学, 工学部, 准教授 (80467448)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ナノ構造形成 / ＤＮＡ１分子操作

Outline of Final Research Achievements

We propose a packaging technology using DNA molecules as scaffolds for realizing high-density and precise arrangement of DNA nano-devices. Single DNA molecules are extended and immobilized between micro-electrodes to form "DNA highwires".
In this project, we focused on a DNA manipulation technique under a fluorescence microscope for forming DNA highwires. We successfully manipulated single DNA molecules using two microstructures driven by two laser beams, where we handled them like ‘chopsticks’. We evaluated the dimensions and the shapes of microstructures, intended for holding single DNA molecules between them efficiently. We have found that the geometry of the structure is dominant parameter for effective handling of single DNA molecules. This manipulation technique allows the imaging of single DNA molecules with its intact state, which will lead to fabrication of DNA nanosystems.

Free Research Field

マイクロ・ナノ工学