Spatio-temporal analysis to elucidate the 2D pattern formation mechanisms

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K13325
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Osaka Prefecture University
• Principal Investigator: Hagiwara Masaya 大阪府立大学, 21世紀科学研究機構, 講師 (00705056)
• Co-Investigator(Kenkyū-buntansha): XU YAN 大阪府立大学, 21世紀科学研究機構, 講師 (90593898)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: 培養制御 / 細胞位置制御 / パターンフォーメーション / 反応拡散モデル

Outline of Final Research Achievements

We have developed in vitro experimental model to generate two-dimensional pattern formation on a glass slide. The cell position control is relatively easy for 2D culture and our system does not require heterotypic cell-cell interactions but epithelial cells can produce the specific pattern by themselves. Here we employed photolithography technologies to control initial culture environments on a glass slide in order to match the in-vitro experimental conditions and simulation conditions exactly. By conducting pattern formation experiments and developmental simulations simultaneously with the same initial cell positions, quality of the analysis can be improved significantly to elucidate the pattern formation mechanisms.

Free Research Field

マイクロナノシステム