Challenge to measure IR spectrum of fluorescent protein chromophore

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13621
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical chemistry
• Research Institution: Okayama University of Science (2016-2017); Tokyo Institute of Technology (2015)
• Principal Investigator: Sakai Makoto 岡山理科大学, 理学部, 教授 (60298172)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 赤外分光 / タンパク質 / 発色団 / 超解像 / フラビン

Outline of Final Research Achievements

IR spectroscopy is one of the powerful tools for investigation of structure of proteins. However, it is difficult to measure IR spectra in aqueous aqueous solution because large IR absorption of water. In this study, we developed a novel transient fluorescence-detected resonance IR spectroscopy, and succeeded the measurement of IR spectrum of flavin mononucleotide, that is one of the fluorescent protein chromosphere, in aqueous solution. Measured spectrum was compared with these measured by Raman spectroscopy. These results will be regarded as the data very useful for the measurement of IR spectrum of fluorescence protein chromosphere.

Free Research Field

物理化学