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2016 Fiscal Year Final Research Report

Emergence of protein-like higher-order structure and molecular recognition ability by dynamic exchange of polypeptide side chains

Research Project

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Project/Area Number 15K13705
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Polymer chemistry
Research InstitutionYokohama National University

Principal Investigator

OYAMA Toshiyuki  横浜国立大学, 大学院工学研究院, 教授 (30313472)

Co-Investigator(Renkei-kenkyūsha) TOKORO Yuichiro  横浜国立大学, 大学院工学研究院, 助教 (80709692)
Research Collaborator INOUE Yuki  
URAI Satoshi  
NAKAGAWA Kazutoshi  
KITAHARA Yukina  
Project Period (FY) 2015-04-01 – 2017-03-31
Keywordsポリペプチド / 動的共有結合 / 高次構造 / タンパク質
Outline of Final Research Achievements

A water-soluble polypeptide having a methyl thioester structure at the side chain was synthesized, and this polypeptide was reacted with various low-molecular-weight thiols in water. As a result, progress of dynamic bond exchange between thioester and thiol was confirmed, which indicates successful rearrangement of the polypeptide side-chain sequence in water. It was also revealed that the higher-order structure of the polypeptide changed with the progress of side-chain exchange. Furthermore, it was shown that when the exchange was carried out by using low-molecular-weight thiols having relatively high solubility in water, the resulting copolypeptide could be isolated while maintaining the side-chain composition and higher-order structure generated during the exchange reaction.

Free Research Field

機能性高分子の合成および応用

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Published: 2018-03-22  

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