Monitoring of association of cell membrane receptors based on the resonance energy transfer through cell membrane

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K13728
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Sueda Shinji 九州工業大学, 大学院情報工学研究院, 准教授 (00325581)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: タンパク質間相互作用 / 細胞膜レセプター / 共鳴エネルギー移動 / テルビウム錯体 / GFP

Outline of Final Research Achievements

In the present study, we attempted to construct a system for monitoring association of cell membrane receptors based on the resonance energy transfer through cell membrane by labeling the receptors with donor and acceptor dyes on the outer and inner surface of cell membrane, respectively. Specifically, bradykinin B2 receptor (B2R) was chosen as a model receptor and it was labeled with a terbium complex and GFP on the outer and inner surface of cell membrane, respectively. With this system, we obtained data suggesting the association of B2R by monitoring the signal based on energy transfer from the terbium complex to GFP.

Free Research Field

生物分析化学