Fine chemical synthesis of poly(ADP-ribose) for disease studies

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K13736
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Okamoto Akimitsu 東京大学, 先端科学技術研究センター, 教授 (60314233)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: ポリADP-リボース / 化学合成 / 固相合成

Outline of Final Research Achievements

This research project was carried out for a year in FY2015. In this year we established the efficient synthetic process of poly(ADP-ribose). Because of the short research term, we concentrated to the synthesis of a linear poly(ADP-ribose) and explored the best suited reaction conditions. Based on a phosphoramidite process, one of DNA solid support synthesis, we created a new chemical synthesis of poly(ADP-ribose). Ribosyladenosine was protected with a dimethoxytrityl group for a hydroxy group to be linked with another building block and benzoyl groups for a the other hydroxy groups. In addition, a ribose unit for a starter and a adenosine unit for a capping were also prepared. We obtained a ADP-ribose dimer through a synthetic cycle.

Free Research Field

生物有機化学