N-Terminally fluorescent-labeled antibodies that show fluorescence change upon antigen-binding

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13739
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: Hohsaka Takahiro 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (30263619)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗体 / イムノアッセイ / 蛍光

Outline of Final Research Achievements

In this study, a novel method was developed for producing N-terminally fluorescent-labeled IgG antibodies that show antigen-dependent fluorescence change. By reacting fluorescent-labeled aldehyde with IgG antibody under weakly acidic condition, the fluorescent group was specifically attached at the N terminus, and the resulting N-terminally fluorescent-labeled antibody showed fluorescence intensity changed upon the antigen binding. In addition, it is possible to synthesize dual-labeled fluorescence-responsive antibody that can detect the antigen-binding as a change in fluorescence intensity ratio, and to detect tagged proteins expressed in a cell-free translation system in a real-time manner.

Free Research Field

生体関連化学