2016 Fiscal Year Final Research Report
Development of novel radical enzymes bearing unusual protein-derived cofactor
| Project/Area Number |
15K13743
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 人工金属酵素 |
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| Outline of Final Research Achievements |
Galactose oxidase and copper amine oxidase have the protein-derived redox-active organic cofactors generated by post-translational modification near the catalytic active center. There are several lines of evidences that the copper ion plays an important role in the formation of these cofactors. The copper-substituted variants of cupin proteins have been prepared by using an E. coli expression system with a copper-containing medium. Furthermore, we introduced Tyr and/or Cys residues into several site surrounding copper center and confirmed the formation of Tyr-Cys by spectroscopic method as well as X-ray crystal structural analysis.
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| Free Research Field |
生体機能関連化学
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