Chemical splicing for regulation of functional nucleic acids and cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13749
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kumamoto University
• Principal Investigator: Ihara Toshihiro 熊本大学, 大学院先端科学研究部(工), 教授 (40253489)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA conjugate / terpyridine / anthracene / sequence edition / metal ion / photo dimerization / omega-shaped structure / chemical splicing

Outline of Final Research Achievements

Two terpyridine units were built into the distal sites of a DNA backbone to obtain the conjugates. The conjugate formed an intramolecular 1:2 complex with some transition metal ions such as Fe3+ and Ni3+ to take an Ω-shaped structure. The two sequences located outsides of two terpyridine units was connected directly with each other to provide a new sequence. That is, the continuity of distal segments was controlled by metal ion-induced Ω-shaped conformation. We succeeded in the activity control of a split DNAzyme using this techniques of metal ion-directed reversible sequence edition.
Two anthracene units were built into the DNA backbone to obtain the conjugates. The conjugate formed an Ω-shaped structure through intramolecular dimerization of anthracene by photoirradiation in the presence of the template DNA carrying the sequence complementary to flanking two sequences of anthracene units. This could be regarded as photo-switchable techniques of reversible sequence edition.

Free Research Field

生物分析化学、核酸化学