Hybrid Polymeric Materials Grafting Stimuli-responsive Near-infrared Dyes and Their Bioapplication

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K13770
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Organic and hybrid materials
• Research Institution: Kyoto University
• Principal Investigator: MIKI KOJI 京都大学, 工学研究科, 准教授 (60422979)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: pH応答性 / 近赤外色素 / シアニン / 蛍光 / 腫瘍

Outline of Final Research Achievements

pH-Responsive near-infrared fluorescent cyanine dyes have been developed. From UV-vis absorption and fluorescence measurements, dyes showed pH-dependent equilibrium between a fluorescent open-ring structure and a non-fluorescent closed-ring structure in the various pH ranges. These behaviors depend on their nucleophilicity of substituents. The pH responsiveness is reversible in all cases. From in vitro cell culture experiments, dyes were found to show the pH-dependent enhancement of fluorescence intensively through endocytosis. PEG-grafted pH-responsive dyes accumulated in tumor sites and visualized them in in vivo optical imaging.

Free Research Field

有機合成化学