Photopolymerization-based 3D Single Cell Spotter for Reconstruction of Tissue Array

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K13910
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Toyohashi University of Technology
• Principal Investigator: nagai Moeto 豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (00580557)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 細胞3Dプリンタ / 単一細胞 / ノズルアレイ / 微小液滴生成 / 多点光照射 / 光硬化性樹脂

Outline of Final Research Achievements

Our objective is to assemble a 3D tissue with a single cell spotter. This study aims to achieve high throughput single cell printing. We made the essential part of a cell spotter. Adjustable openings were composed of SU-8 vertical openings and PDMS pneumatic valves. We fabricated a nozzle structure and designed flow channels to trap single cells on the openings by calculating flow ratio through microchannels. The structure was assembled from a two-step SU-8 structure and PDMS channel. These fundamental fabrication technologies enable the patterning of single cells on a substrate. Photocurable resin droplets were generated in a microchannel and its properties were characterized.

Free Research Field

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