2017 Fiscal Year Final Research Report
Extraction of cellular component without killing cells
| Project/Area Number |
15K14235
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
馬越 大 大阪大学, 基礎工学研究科, 教授 (20311772)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 脂質膜場 / DNA / RNA / 抽出 / 膜タンパク質 / 電気泳動 |
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| Outline of Final Research Achievements |
This research purpose is the development of pretreatment process, which enables the specific biomolecular recognition and the extraction of specific cellular components. To attain this purpose, selective labeling of cell membrane was possible with hydrophobic quantum dots. Furthermore, high sensitive detection of biomolecules was attained by surface enhanced Raman analysis and in situ nano-particle preparation. As a extraction and separation, an easy biomolecular extraction method was attained with fatty acid immobilized beads. Furthermore, chiral recognition ability was induced by polymerization of polymerizable lipid and chiral selective adsorption was attained. To further purify and analyze membrane proteins, electrophoretic separation method in lipid nano membrane was attained in nature state. Thus, Our developed method would attribute to single cell analysis.
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| Free Research Field |
分析化学
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