Development of novel artificial biocatalyst for carbon dioxide to methanol conversion due to multi-electrons-protons coupling reduction

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14239
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka City University
• Principal Investigator: AMAO YUTAKA 大阪市立大学, 複合先端研究機構, 教授 (80300961)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 二酸化炭素還元 / 生体触媒 / ビオローゲン / 人工補酵素 / 人工光合成 / メタノール

Outline of Final Research Achievements

In this proposal, to develop the novel catalyst for carbon dioxide conversion to methanol, formate, aldehyde and alcohol dehydrogenase hybrid biocatalysts system is designed. The interaction with alcohol dehydrogenase catalyzing the formaldehyde-methanol conversion and single-electron reduced methylviologen was investigated using enzymatic kinetic analysis. The interaction with aldehyde dehydrogenase catalyzing the formic acid-formaldehyde conversion and single-electron reduced methylviologen also was investigated using enzymatic kinetic analysis. As a result of enzymatic kinetic analysis, the interaction with aldehyde dehydrogenase shows stronger affinity for single-electron reduced methylviologen than other dehydrogenases. Based on these results, carbon dioxide-methanol conversion by formate, aldehyde, alcohol dehydrogenases system with single-electron reduced methylviologen was achieved.

Free Research Field

生体触媒化学