Cell-type-specific patch-clamp recordings from deep brain of awake behaving mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14313
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Nagoya University
• Principal Investigator: Yamashita Takayuki 名古屋大学, 環境医学研究所, 助教 (40466321)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: パッチクランプ / 大脳皮質 / マウス

Outline of Final Research Achievements

To understand the neuronal mechanisms underlying animal behavior, it is essential to perform electrophysiological measurements of membrane potential dynamics of neurons in awake behaving animals with a high temporal resolution in a cell-type specific manner. In this study I verified technical feasibility of cell-type (or projection-target) specific patch-clamp recordings combined with optogenetics using task-performing mice. I successfully recorded membrane potentials of specific cell-types in multiple layers of primary somatosensory cortex of mice exhibiting spontaneous behavior or performing a simple learning task and addressed important questions on neuronal processing of whisker-related tactile information.

Free Research Field

神経生理学