2016 Fiscal Year Final Research Report
New infection directivity by modification of capsid proteins of adeno-associated virus.
| Project/Area Number |
15K14333
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 神経科学 / 神経回路網 / アデノ随伴ウイルス / キャプシド / 特異的感染 |
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| Outline of Final Research Achievements |
We develop new technologies that allow neural cell type-specific and neural pathway-specific gene transduction with viral vector alone by modification of AAV capsid proteins. The strategy is simple, and we pursued research based on the following two policies. (i) Removal of nonspecific infectivity: it was confirmed by cultured cells that infectivity was dramatically decreased by replacing various amino acid sequences prescribing extensive infection directivity. Subsequently, verification is underway with primary cultured neurons and adult mice. (ii) Granting new infectivity: we aim to acquire new infectivity by adding / inserting specific sequence to capsid protein. Candidate specific sequences were prepared and purified. We are proceeding with verification in adult mouse.
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| Free Research Field |
神経解剖学
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