Establishment of efficient induction system for primordial germ cells from ES cells in vitro

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14365
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kyoto University
• Principal Investigator: Ohta Hiroshi 京都大学, 医学研究科, 助教 (50391892)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 始原生殖細胞 / ES細胞 / EpiLC / 分化誘導

Outline of Final Research Achievements

We have succeeded in inducing functional PGCLCs (primordial germ cell-like cells) from ESCs in vitro. In this research, we analyzed the procedure of PGCLC formation to enhance the induction rate of PGCLCs. We identified heterogeneity in EpiLCs (epiblast-like cells), a precursor of PGCLCs, and found that the induction rate of PGCLCs was affected by mouse genetic background. Our findings will provide an improved procedure for inducing PGCLCs from ESCs in vitro.

Free Research Field

生殖生物学